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Endocytic Trafficking Routes of Wild Type and F508 - CiteSee
external epitope-tagged constructs to elucidate the itinerary and kinetics of wild type and F508 CFTR in the endocytic pathway and visualized . because even minor changes in CFTR sequence tend to compromise its folding to a native state (Riordan, 1999). Howard et al. (1995, 2000) inserted a FLAG epitope into the.
A Stable Human-Cell System Overexpressing Cystic Fibrosi
Trial purification using dodecylmaltoside for membrane protein extraction reproducibly recovered 178 ± 56 μg SUMO*-CFTRFLAG-EGFP per billion cells at 80% .. Protein fractions (biotinylated protein or Triton X-100 cell extracts) were spiked with the L-leucine isotope-labeled cell lysate, and then subjected to SDS-PAGE,
ΔF508 CFTR Pool in the Endoplasmic Reticulum Is Increased b
The cystic fibrosis mutation (delta F508) does not influence the chloride channel activity of CFTR. Nat. Genet. 3, 311-316. [PubMed]; Loo, M.A., Jensen, T.J., Cui, L., Hou, Y., Chang, X.B., and Riordan, J.R. (1998). Perturbation of Hsp90 interaction with nascent CFTR prevents its maturation and accelerates its degradation by